thdF [2017-8-11 16:0:3]

GTP-binding protein, putative tRNA modification GTPase

Locus: BSU41020

Isoelectric point: 4.64

Molecular weight: 50.81 kDa

Protein length: 459 aa

Gene length: 1377 bp

Function: tRNA modification

Product: putative tRNA modification GTPase

Essential: no

Synonyms: mnmE, trmE

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation
• Groups of genes → GTP-binding proteins

Gene

Coordinates: 4,211,510 → 4,212,889

Phenotypes of a mutant

• inactivation of thdF reduces sporulation efficiency to 21.9% that of wild type cells; longer mother cells, some with aberrant septa and forespores PubMed

The protein

Catalyzed reaction/ biological activity

• Binds and hydrolyzes GTP and readily exchanges GDP for GTP

Protein family

• MnmE subfamily (according to Swiss-Prot) Era/Obg family

Paralogous protein(s)

• Era, Obg, YphC, YsxC, RbgA, YqeH, ThdF, YyaF, YnbA

Structure

• 1XZP (from Thermotoga maritima, 44% identity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• Information on the corresponding protein from E. coli: EcoCyc
• MnmE required for wild-type 5-methylaminomethyl-2-thiouridine modification of tRNA, interacts with GidA. MnmE also appears to play a role in oxidation of thiophene and furan compounds and regulates glutamate-dependent acid resistance.

Expression and Regulation

Operons

• Genes: thdF-gidA-gidB-noc
  Description: PubMed

  Regulatory mechanism

  • ComK: activation, PubMed, in ComK regulon
  • DnaA: repression, PubMed, in DnaA regulon

  Regulation

  • constitutively expressed PubMed

Biological materials

Mutant

• MGNA-B877 (thdF::erm), available at the NBRP B. subtilis, Japan

Labs working on this gene/protein

• Naotake Ogasawara, Nara, Japan

References

Reviews

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Original publications

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